Masoume Manteghi; Morteza Mamouei; Saleh Tabatabaei Vakili; Jamal Fayazi; Khalil Mirzadeh
Volume 18, Issue 2 , June 2016, , Pages 367-375
Abstract
The Effect of various amount of soybean lecithin replaced with egg yolk to protect the ram semen in times of one hour after semen collection, as well as 24 and 48 hours after the semen store at 5°C was evaluated. Semen was collected weekly for 8 weeks from 10 rams with 2-3 years old and average body ...
Read More
The Effect of various amount of soybean lecithin replaced with egg yolk to protect the ram semen in times of one hour after semen collection, as well as 24 and 48 hours after the semen store at 5°C was evaluated. Semen was collected weekly for 8 weeks from 10 rams with 2-3 years old and average body weights of 64 kg. Semen samples were pooled and divided into 4 parts. Each part was diluted with one of the tris base extender containing 0.5, 1 and 1.5 percent of soybean lecithin and 14 percent of egg yolk. The motility, viability, morphological abnormalities and membrane integrity of spermatozoa and pH of semen were evaluated. The results showed that 1.5 percent of soybean lecithin improved the motility and viability of spermatozoa at the first time (P< 0.05). At the 48 hours after the semen store in 1 and 1.5 percent of soybean lecithin the sperm viability improved when compared with egg yolk treatment (P<0.05). Overall, soybean lecithin is the appropriate replacement with egg yolk and improved the semen quality in times of study. With regard to limitations of egg yolk using with animal source in ram extender, it was recommended to be using the soybean lecithin as the plant source.